Ki67

Purpose

Detection and quantification of Ki-67 protein in various cancers including human prostate and breast cancers.

Services

AQUA staining multiplexed with cytokeratin

Introduction

The Ki-67 protein is a cellular marker for proliferation with a molecular weight of 395 kDa. It is strictly associated with cell proliferation. Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labelling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis.

MIB-1 is a commonly used monoclonal antibody that detects the Ki-67 antigen. It is used in clinical applications to determine the Ki-67 labelling index. It can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval.

 

Specificity

Human tissue. This antibody cross-reacts with the Ki-67-equivalent protein in various mammals, including cow, dog, horse, sheep, and swine.

Staining Interpretation

During the interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).

Ki67, Human tonsil

DCIS

Thomas Jefferson Kimmel Cancer Center

Rui Lab